Rome: Total War - Alexander will allow users to re-create the leader's conquests with a new campaign map and six new historical battles that attempt to re-create the conditions and tactics used in his battles with Greek, Persian, and Indian forces. The campaign adds more than 60 new units and four new factions to the game, two of which will be the Macedonians and Persians.

Over the course of the historical battles, a series of movies featuring the voice work of Brian Blessed (Prince Vultan from Flash Gordon, and the wrestling trainer from Alexander) will tell the story of Alexander and set the stage for each battle.